Assay Time 3 hours
Sensitivity 6.5 pg/mL
Shipping Conditions Ice packs
Latin name Mus musculus
Detection Method Colorimetric
Detection Range 15.6-1000 pg/mL
Estimated Turnaround Time 6-11 Business days
Species Reactivity Mus musculus (Mouse)
Assay Type Double-antibody Sandwich
Research Area Cytokine, Infection immunity
Target Name Colony Stimulating Factor 1, Macrophage
Storage Temperature Short term: 4°C; Long term: see manual.
Application For research use only. Not for diagnostic procedures.
Precaution of Use The Stop Solution is acidic. Do not allow to contact skin or eyes.
Quality Systems The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Target's alterntive name CSF1, M-CSF, Lanimostim, Processed macrophage colony-stimulating factor 1
Shelf life Use Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit before 8 months
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Specificity This assay has high sensitivity and excellent specificity for detection of Colony Stimulating Factor 1, Macrophage
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Cross Activity No significant cross-reactivity or interference between Colony Stimulating Factor 1, Macrophage and analogues was observed.
Test Principle No significant cross-reactivity or interference between Colony Stimulating Factor 1, Macrophage and analogues was observed.
Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 1, Macrophage were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 1, Macrophage were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Description Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.