Rat Colony Stimulating Factor 1, Macrophage (MCSF) ...

Assay Time 3 hours

Sensitivity 3.3 pg/mL

Shipping Conditions Ice packs

Detection Method Colorimetric

Detection Range 7.81-500 pg/mL

Latin name Rattus norvegicus

Estimated Turnaround Time 6-11 Business days

Species Reactivity Rattus norvegicus (Rat)

Assay Type Double-antibody Sandwich

Research Area Cytokine, Infection immunity

Target Name Colony Stimulating Factor 1, Macrophage

Storage Temperature Short term: 4°C; Long term: see manual.

Application For research use only. Not for diagnostic procedures.

Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Precaution of Use The Stop Solution is acidic. Do not allow to contact skin or eyes.

Quality Systems The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Target's alterntive name CSF1, M-CSF, Lanimostim, Processed macrophage colony-stimulating factor 1

Shelf life Use Rat Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit before 8 months

Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids

Specificity This assay has high sensitivity and excellent specificity for detection of Colony Stimulating Factor 1, Macrophage

Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Cross Activity No significant cross-reactivity or interference between Colony Stimulating Factor 1, Macrophage and analogues was observed.

Test Principle No significant cross-reactivity or interference between Colony Stimulating Factor 1, Macrophage and analogues was observed.

About Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 1, Macrophage were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 1, Macrophage were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Description Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.