Target Name NA
Species Reactivity Rat
Target's alterntive name MCSF
Assay Type Sandwich
Assay Time 4.5 hours
Shipping Conditions Ice packs
Sensitivity 9.38 pg/mL
Detection Method Colorimetric
Detection Range 15.63-1000 pg/mL
Latin name Rattus norvegicus
Estimated Turnaround Time 6-11 business days
Sample Type Serum, Plasma, Biological Fluids
Research Area Cancer, Cardiovascular, Immunology
Storage Temperature Short term: 4°C; Long term: see manual.
Application For research use only. Not for diagnostic procedures.
Quality Systems The kit is manufactured at ISO 9001 certified facilities.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Cross Activity No significant cross-reactivity or interference was observed.
Precaution of Use The Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf life Use Rat MCSF (Macrophage Colony Stimulating Factor 1) ELISA Kit before 6 months
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Description Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
Test Principle This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat M-CSF . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat M-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat M-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat M-CSF. The concentration of Rat M-CSF in samples can be calculated by comparing the OD of the samples to the standard curve.