anti-M-CSF Antibody

NCBI GENE nr 1435

GENE symbol CSF1

Works with Human

Ab raised in Rabbit

Protein nr P09603

Ncbi nr P09603

French translation anticorps

Omim nr 311033367

Type Polyclonal

Vial with antibody Lyophilized

Source Homo sapiens

Antibodie's label unlabelled serum

Immuno application ELISA, Western Blot, NELISAut

Antigene name colony stimulating factor 1 (macrophage)

Gene names MCSF, CSF-1Macrophage colony-stimulating factor 1, Lanimostim, CSF-1

Antigen Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hM-CSF (human M-CSF).

Purity Anti-hM-CSF specific antibody was purified by affinity chromatography employing immobilized hM-CSF matrix.

Properties If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Keep at M-CSF antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.

Gene info Macrophage CSF, also known as CSF1, is produced by monocytes, fibroblasts and endothelial cells. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osetoclasts. Natural human MCSF is a dimeric glycoprotein of 70-90 kD molecular weight, existing in multiple glycosylation forms. It binds to a 165 kD glycoprotein of the receptor tyrosine kinase subclass III, a family that includes the receptors for platelet derived growth factor (PDGF) and stem cell factor (SCF).

Antibody advice Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/mL), a concentration of 0.02 0.03 ug/mL of this antibody is required. ELISA:To detect hM-CSF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF. Sandwich:To detect hM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human M-CSF (XP-5227Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hM-CSF. Western Blot:To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.