anti-M-CSF Antibody

GENE symbol CSF1

NCBI GENE nr 1435

Works with Human

Antibodie's label Biotin

Ab raised in Rabbit

Protein nr P09603

Ncbi nr P09603

French translation anticorps

Omim nr 311033367

Type Polyclonal

Vial with antibody Lyophilized

Source Homo sapiens

Immuno application ELISA, Western Blot

Antigene name colony stimulating factor 1 (macrophage)

Gene names MCSF, CSF-1Macrophage colony-stimulating factor 1, Lanimostim, CSF-1

Purity Anti-hM-CSF specific antibody was purified by affinity chromatography and then biotinylated.

Antigen Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hM-CSF (human Granulocyte Colony Stimulating Factor).

Properties If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Keep at M-CSF antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.

Gene info Four distinct colony-stimulating factors (CSFs) that promote survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte macrophage CSF (GMCSF), granulocyte CSF (GCSF), macrophage CSF (MCSF), and Interleukin-3 (IL-3, Multi CSF). Both GMCSF and IL-3 are multipotential growth factors, stimulating proliferation of progenitor cells from more than one hematopoietic lineage. In contrast, GCSF and MCSF are lineage restricted hematopoietic growth factors, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors.

Antibody advice ELISA:To detect hM-CSF by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 ug/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hM-CSF.Sandwich:To detect hM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human M-CSF (XP-5227) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hM-CSF. Western Blot:To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.