anti-M-CSF Antibody

Ab raised in Goat

GENE symbol Csf1

Works with Mouse

NCBI GENE nr 12977

Protein nr P07141

Antibodie's label Biotin

Ncbi nr P07141

Omim nr 1345843

French translation anticorps

Type Polyclonal

Vial with antibody Lyophilized

Source Mus musculus

Immuno application ELISA, Western Blot

Antigene name colony stimulating factor 1 (macrophage)

Gene names op, Csfm, MCSF, C87615, Macrophage colony-stimulating factor 1CSF-1

Purity Anti-mM-CSF specific antibody was purified by affinity chromatography and then biotinylated.

Antigen Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mM-CSF (murine M-CSF).

Properties If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Keep at M-CSF antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.

Antibody advice ELISA:To detect mM-CSF by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 ug/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mM-CSF. Sandwich:To detect mM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine M-CSF (XP-5228) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mM-CSF.Western Blot:To detect mM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.