NCBI ID 1435
Protein's origin Human
Recombinant in E. coli
Rec. PROTEIN GI# 27262661
His tag on the recombinant protein no His tag
Accession nr NP_000748.3
Source Homo sapiens
Additional source Recombinants or rec. proteins
About This product is for research use only.
Gene name colony stimulating factor 1 (macrophage)
Pureity Greater than 98% by SDS-PAGE gel and HPLC analyses.
Other name of the recombinant MCSF, CSF-1, Macrophage colony-stimulating factor 1, Lanimostim
SEQUENCE MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
Activity The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is = 1.0 ng/mL, corresponding to a specific activity of = 1 x 106 units/mg.
Keep at The lyophilized M-CSF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted M-CSF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing M-CSF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
About recombinant protein M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant human M-CSF is a 36.8 kDa homodimeric protein containing two 159 amino acid polypeptide subunits.